Assignment: Upload the lab report of lab test
Dear student,
the assignment is aligned with:
- Obj. 1 Be able to perform the following biochemical tests and understand how they work: carbohydrate fermentation, gelatin hydrolysis, starch hydrolysis, casein hydrolysis, urea hydrolysis, citrate utilization, catalase activity, tryptophan hydrolysis (indole test). (C.O. # 4 Identify unknown bacteria using various physiological tests.)
- Obj. 2 Be able to relate the results of the tests performed to the different types of metabolic activities carried out by individual bacterial species. (C.O. # 4 Identify unknown bacteria using various physiological tests.)
Assignment:
Upload the lab report of the actual assigned
lab test that your instructor assigned you to perform (to be announced).
The instructor will determine which lab test you will actually conducting in the lab from the book Module “LAB 10: BIOCHEMICAL TESTING”
Upload the lab report of the actual assigned lab test that your instructor assigned you to perform.
The structure of the lab report depends on requirements of the instructor.
Motivation:
Motivation for the lab report is that students experience and demonstrate the skills 1) to apply the scientific method, 2) apply standard techniques in microbiology, 3) process and understand the underlying theoretical concepts of microbiology.
Instruction:
One format for a lab report is to report the 1) title of the experiment or test, 2) the hypothesis or research question of the experiment or test, 3) the prediction, 4) the procedure, 5) the results, and 6) the conclusion.
Example of a lab report -
1) title of the experiment or test: Catalase test
2) the hypothesis or research question of the experiment or test: It is hypothesized that the bacteria to be tested will be catalase positive OR the research question studied is whether or not the tested bacteria are calatase positive
3) the prediction: If the bacteria are catalase positive, then formation of bubbles will be observed during the test
4) the procedure: Transfer bacteria of interest onto microscopic slide using aseptic techniques. Apply enough droplets of 3% hydrogen peroxide solution, so that microbes are covered. Observe microbes in 3% hydrogen peroxide solution for 1 to 3 minutes for the formation of bubbles. Document the results. In parallel, include a positive control of a known lab strain that is positive for catalase
5) the results:
The bacteria of interest did/ did not form bubbles (circle appropriate result).
The positive control did/did not form bubbles (circle appropriate result).
Include photos if available.
6) the conclusion:
The positive control did/did not work , because the bubbles were/were not formed (circle appropriate result). Therefore, the test reagents and the test itself is/is not working properly (circle appropriate result).
The unknown/bacteria of interest are/are not catalase positive, because the bacteria did/did not form bubbles (circle appropriate result). The results do/don’t support the hypothesis (circle appropriate result).
You can upload your report as a file, or write by hand on a piece of paper and upload a photo of your handwritten report.
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